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Contents of README:PerlPrimer is a free, open-source GUI application written in Perl that designs primers for standard PCR, bisulphite PCR, real-time PCR (QPCR) and sequencing. It aims to automate and simplify the process of primer design. PerlPrimer's current features include the following: - Calculation of possible primer-dimers - Retrieval of genomic or cDNA sequences from Ensembl (including both sequences automatically for QPCR) - Ability to BLAST search primers using the NCBI server or a local server - Results can be saved or optionally exported in a tab-delimited format that is compatible with most spreadsheet applications. - ORF and CpG island detection algorithms - Ability to add cloning sequences to primers, automatically adjusted to be in-frame - QPCR primer design without manual intron-exon boundary entry An optional run-time dependency is spidey, for Real Time PCR. For details and citation: Marshall OJ. PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR. Bioinformatics 2004 20(15):2471-2472 |
Name Last modified Size
Parent Directory -
README 14-Jul-2018 02:06 1.0K
References 26-Nov-2013 10:57 158
doinst.sh 26-Nov-2013 10:57 129
perlprimer.SlackBuild 03-Aug-2019 02:58 2.5K
perlprimer.desktop 14-Jun-2018 16:00 126
perlprimer.info 03-Aug-2019 02:58 393
perlprimer.png 05-Sep-2011 00:00 4.8K
slack-desc 14-Jul-2018 02:06 936
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